Global Identification of Protein Post-translational Modifications in a Single-Pass Database Search

نویسندگان

  • Michael R. Shortreed
  • Craig D. Wenger
  • Brian L. Frey
  • Gloria M. Sheynkman
  • Mark Scalf
  • Mark P. Keller
  • Alan D. Attie
  • Lloyd M. Smith
چکیده

Bottom-up proteomics database search algorithms used for peptide identification cannot comprehensively identify post-translational modifications (PTMs) in a single-pass because of high false discovery rates (FDRs). A new approach to database searching enables global PTM (G-PTM) identification by exclusively looking for curated PTMs, thereby avoiding the FDR penalty experienced during conventional variable modification searches. We identified over 2200 unique, high-confidence modified peptides comprising 26 different PTM types in a single-pass database search.

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عنوان ژورنال:

دوره 14  شماره 

صفحات  -

تاریخ انتشار 2015